A Comparative Analysis of Choorna and Bhavitha Choorna of Flower Buds of Japa (Hibiscus rosa-sinensis Linn.) through High-performance Thin-layer Chromatography

Abstract

Introduction:High-performance thin-layer chromatography (HPTLC) is nowadays adopted by the major  pharmacopoeias of the world for analysis of herbal drugs and preparations. The current use is generally limited to  the visual observation of the fingerprints for identification and detection of adulterations and falsifications. It makes  sense to use HPTLC to expand chromatographic fingerprints to identify the main active ingredients in medicinal  plants. Compared to thin layer chromatography, the separation and resolution are significantly superior, and the  outcomes are far more consistent and repeatable. Materials and Methods: Japakusuma mukula (flower buds of Japa) which is botanically identified as Hibiscus rosa sinensis Linn. contains various phytoconstituents such as flavonoids, terpenoids, steroids, polysaccharides, alkaloids,  amino acids, lipids, sesquiterpene, quinones, and naphthalene groups. HPTLC fingerprinting profile of choorna (powder) and bhavitha choorna (triturated powder) of flower buds of Hibiscus rosa-sinensis Linn. is demonstrated in  methanolic extract in this study and bands are analyzed at 254 nm and 366 nm using CAMAG Linomat V Automatic  Sample Spotter. The peaks and area are compared. Results and Conclusion: Similarity in the peak areas even in different Max Rf values, it could be inferred that  the phytoconstituent obtained in both conditions will be having similarity. Since the bhavitha choorna possess, the  greater number of large value peak areas in both the wavelength of visualization, it is clear that bhavitha choorna is  more potent in comparison with choorna.