Standardization of Gunja Taila

Abstract

Sneha kalpana is one of the unique and commonly prescribed Ayurvedic dosage form in  day-to-day practice having increase potency, palatability, shelf life etc. Although varities  of Snehas are described in Ayurvedic texts, the most common amongst them are Taila &  Ghrita kalpana. The research work entitled “Standardization of Gunja Taila” was  planned to standardize two formulations as per the reference of Chakradatta and  Bhaishajya Ratnavali. Therefore, two samples of Gunja Taila with their three batches  each, were prepared with their conceptual references. Both the samples were analyzed  based on Organoleptic Parameters, i.e., Appearance, Colour, Odour, Touch, etc. and  Physico-chemical Parameters i.e., Foreign Matter, Specific gravity, Saponification Value,  Peroxide Value, Baudouin’s Test, Mineral oil Test, Viscosity, Total fatty Matter,  Refractive Index, Brix Value, Rancidity, Optical Rotation, Acid Value, Iodine Value. The  findings for these parameters were found to be satisfactory for quality assurance. Assay of  heavy metals (Pb, Cd, As, Hg), Total bacterial count and Total fungal count are under the  permissible limit depicted in the study. Specific pathogens and aflatoxins (B1, B2, G1, G2)  are absent in all samples. HPTLC is an important tool to generate the standardization  parameter for quality control purpose. Sample GT-1 and GT-2 were analysed on different  wavelengths 510 nm, 366 nm (Long) & 254 nm (Short). It confirmed 11 distinct spots at  510 nm, 06 distinct spots at 366 nm and 12 spots at 254 nm of GT-1 samples whereas 12  distinct spots at 510 nm, 06 distinct spots at 366 nm and 12 spots at 254 nm were observed  in GT-2 samples. The Rf values of spots differentiate the chemical composition of the  sample.